A new method to identify flanking sequence tags in chlamydomonas using 3’-RACE

@inproceedings{MesletCladire2012ANM,
  title={A new method to identify flanking sequence tags in chlamydomonas using 3’-RACE},
  author={Laurence Meslet-Cladi{\'e}re and Olivier Vallon},
  booktitle={Plant Methods},
  year={2012}
}
The green alga Chlamydomonas reinhardtii, although a premier model organism in biology, still lacks extensive insertion mutant libraries with well-identified Flanking Sequence Tags (FSTs). Rapid and efficient methods are needed for FST retrieval. Here, we present a novel method to identify FSTs in insertional mutants of Chlamydomonas. Transformants can be obtained with a resistance cassette lacking a 3’ untranslated region (UTR), suggesting that the RNA that is produced from the resistance… CONTINUE READING
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