A new method to extract nuclei from paraffin-embedded tissue to study lymphomas using interphase fluorescence in situ hybridization.

@article{Paternoster2002ANM,
  title={A new method to extract nuclei from paraffin-embedded tissue to study lymphomas using interphase fluorescence in situ hybridization.},
  author={Sarah F Paternoster and Stephanie R Brockman and Rebecca F. McClure and Ellen D. Remstein and Paul James Kurtin and Gordon W. Dewald},
  journal={The American journal of pathology},
  year={2002},
  volume={160 6},
  pages={1967-72}
}
Fluorescence in situ hybridization (FISH) is difficult to accomplish using thin-sections of paraffin-embedded lymphoid tissue because of the high cellularity and truncated cells that interfere with accurate scoring of individual nuclei. We modified and tested a new technique to isolate individual nuclei from tissue cores of paraffin-embedded tissue… CONTINUE READING