A new method for simultaneous gene deletion and down-regulation in Brucella melitensis Rev.1.

  title={A new method for simultaneous gene deletion and down-regulation in Brucella melitensis Rev.1.},
  author={Ali Reza Saeedinia and Mehdi Zeinoddini and Masoud Soleimani and Majid Sadeghizadeh},
  journal={Microbiological research},
2 Citations
Improvement of the attenuated mutant strain of Brucella melitensis Rev1 as a potential vaccine candidate
This kanR-free Rev1 mutant strain lack the O-polysaccharide and have a reduced ability to enter and survive in the host cell, and could be evaluated as a potential vaccine candidate if eliciting protective immunity.
Designing and Construction of the Suicide Vector pDS132-ΔkanR to Delete Kanamycin Resistance Sequence in Mutant Strain of Brucella melitensis Rev1 Mutant Strain in order to Generate a Candidate Vaccine Strain
Design and construction of pDS132Δkan suicide vector to delete kanamycin resistance sequence (kan) in a mutant strain of Brucella melitensis rev1 to generate a candidate vaccine strain is designed and construction.


Integration host factor is involved in transcriptional regulation of the Brucella abortus virB operon
VirB expression analyses carried out using lacZ transcriptional fusions showed that virB promoter (PvirB) is temporally activated within J774 cells, and IHF plays a key role during intracellular virB operon expression being required for the biogenesis of the endoplasmic reticulum‐derived replicative vacuole.
Identification of the Perosamine Synthetase Gene ofBrucella melitensis 16M and Involvement of Lipopolysaccharide O Side Chain in BrucellaSurvival in Mice and in Macrophages
The results suggested that S-LPS or, more precisely, its O side chain is essential for survival in mice but not in macrophages.
A Homologue of an Operon Required for DNA Transfer in Agrobacterium Is Required in Brucella abortusfor Virulence and Intracellular Multiplication
Mouse infection assays demonstrated that the virB operon constitutes a major determinant of B. abortus virulence, and it is suggested that putative effector molecules secreted by this type IV secretion system determine routing of B., abortus to an endoplasmic reticulum-related replication compartment.
Differential Requirements for VirB1 and VirB2 during Brucella abortus Infection
The nonpolar virB2 mutant was unable to cause persistent infection in the mouse model, demonstrating the essential role of VirB2 in the function of the T4SS apparatus during infection.
Activity of native vs. synthetic promoters in Brucella.
A set of broad-host-range vectors expressing the lacZ reporter gene from various promoters demonstrates the usefulness of synthetic promoters for enhanced level of gene expression in Brucella spp.
The Brucella suis virB operon is induced intracellularly in macrophages
Fluorescence-activated cell sorter analysis and fluorescence microscopy showed that the virB promoter is induced in macrophages within 3 h after infection, and phagosome acidification was shown to be the major signal inducing intracellular expression.
Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system
The results suggest that VjbR co‐ordinates expression of the T4SS and at least two of its secreted substrates.
Comparative Proteomics Analyses Reveal the virB of B. melitensis Affects Expression of Intracellular Survival Related Proteins
Data indicated that the virB operon may control the intracellular survival of Brucella by affecting the expression of relevant proteins, particularly those involved in amino acid transport and metabolism, lipid metabolism, energy production, cell membrane biogenesis, translation, post-translational modifications and protein turnover.