A new method for simultaneous gene deletion and down-regulation in Brucella melitensis Rev.1.

@article{Saeedinia2015ANM,
  title={A new method for simultaneous gene deletion and down-regulation in Brucella melitensis Rev.1.},
  author={Ali Reza Saeedinia and Mehdi Zeinoddini and Masoud Soleimani and Majid Sadeghizadeh},
  journal={Microbiological research},
  year={2015},
  volume={170},
  pages={
          114-23
        }
}
2 Citations
Improvement of the attenuated mutant strain of Brucella melitensis Rev1 as a potential vaccine candidate
TLDR
This kanR-free Rev1 mutant strain lack the O-polysaccharide and have a reduced ability to enter and survive in the host cell, and could be evaluated as a potential vaccine candidate if eliciting protective immunity.
Designing and Construction of the Suicide Vector pDS132-ΔkanR to Delete Kanamycin Resistance Sequence in Mutant Strain of Brucella melitensis Rev1 Mutant Strain in order to Generate a Candidate Vaccine Strain
TLDR
Design and construction of pDS132Δkan suicide vector to delete kanamycin resistance sequence (kan) in a mutant strain of Brucella melitensis rev1 to generate a candidate vaccine strain is designed and construction.

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