A new method for sequencing DNA.

  title={A new method for sequencing DNA.},
  author={Allan M. Maxam and Walter Gilbert},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={74 2},
  • A. Maxam, W. Gilbert
  • Published 1 February 1977
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the… Expand
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