A new method for protein coexpression in Escherichia coli using two incompatible plasmids.

@article{Yang2001ANM,
  title={A new method for protein coexpression in Escherichia coli using two incompatible plasmids.},
  author={Wenhua Yang and Lei Zhang and Z G Lu and William Tao and Zhong He Zhai},
  journal={Protein expression and purification},
  year={2001},
  volume={22 3},
  pages={472-8}
}
It is commonly believed that incompatible plasmids carrying the same replicon cannot coexist stably in one Escherichia coli cell. However, we found that two incompatible plasmids carrying different antibiotic resistance genes, if under the selection pressure of the two antibiotics, can coexist in E. coli for at least 14 h, which is adequate for routine culture and protein expression. Based on this discovery, we developed a new method to coexpress foreign proteins in E. coli using two… CONTINUE READING

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