A new method for permeabilization of cultured cells without cell damage.

@article{Shimizu1996ANM,
  title={A new method for permeabilization of cultured cells without cell damage.},
  author={Norio Shimizu and Y Kawazoe},
  journal={Biological & pharmaceutical bulletin},
  year={1996},
  volume={19 3},
  pages={484-6}
}
Transient permeabilization was readily achieved by vortex-stirring cultured mammalian cells for a few seconds in the presence of 1 to 10 micrograms/ml of high molecular weight polyacrylic acid (PAA). This was demonstrated by an appreciable uptake by murine leukemia L1210 cells of Lucifer Yellow (LY), which is known to be a non-permeant except under conditions in which micropores are induced in the plasma membrane. In addition, the cytotoxicity of bleomycin (BLM), a very poor permeant, was… CONTINUE READING

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