A new method for on-line measurement of the volumetric oxygen uptake rate in membrane aerated animal cell cultures.

@article{Ducommun2000ANM,
  title={A new method for on-line measurement of the volumetric oxygen uptake rate in membrane aerated animal cell cultures.},
  author={Paul Ducommun and Pascal Ruffieux and Mari Furter and Ian William Marison and Urs von Stockar},
  journal={Journal of biotechnology},
  year={2000},
  volume={78 2},
  pages={139-47}
}
Oxygen is a key substrate in animal cell metabolism and its consumption is thus a parameter of great interest for bioprocess monitoring and control. A system for measuring it based on an oxygen balance on the liquid phase was developed. The use of a gas-permeable membrane offered the possibility to provide the required quantity of oxygen into the culture, while avoiding problems of foaming or shear stress generally linked to sparging. This aeration system allowed moreover to keep a known and… CONTINUE READING