A new method for multi-site-directed mutagenesis.

  title={A new method for multi-site-directed mutagenesis.},
  author={Jing Tian and Qiong Liu and Sheng Dong and Xifeng Qiao and Jiazuan Ni},
  journal={Analytical biochemistry},
  volume={406 1},
A modified method for multi-site-directed mutagenesis was developed here based on polymerase chain reaction (PCR), DpnI digestion, and overlap extension. It needs only methylated plasmids obtained by Dam methyltransferase or plasmids from dam(+)Escherichia coli containing target gene. The procedure consists of PCR, DpnI digestion, overlap extension PCR, and plasmid transformation. The method was developed for multi-site-directed mutagenesis, including close proximity of mutation sites. It does… CONTINUE READING
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PCRmediated deletion of plasmid DNA

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