A new method for measuring spectral absorption coefficients of marine particles

@article{Allali1995ANM,
  title={A new method for measuring spectral absorption coefficients of marine particles},
  author={Karima Allali and Annick Bricaud and Marcel Babin and Andr{\'e} Morel and Patrick Chang},
  journal={Limnology and Oceanography},
  year={1995},
  volume={40},
  pages={1526-1532}
}
A new method has been developed to measure the light absorption coefficients of marine particles. The procedure, adapted from one proposed earlier for microscopic observation of nanoplankton, consists of concentrating particles onto a Nuclepore filter, transferring the filtered material to a glass microscope slide using liquid nitrogen freezing, and finally measuring the particle absorption spectrum on the slide. Measurements on various phytoplanktonic species show that the transfer efficiency… 
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The present paper briefly reviews some of the methodological issues for measuring light absorption by aquatic particles and recommends recommendations for improving the measurements.

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25 References

Algorithms for determining the absorption coefficient for aquatic particulates using the quantitative filter technique

  • B. Mitchell
  • Environmental Science
    Defense, Security, and Sensing
  • 1990
Particulates in aquatic environments dominate variability in inherent and apparent optical properties. Dilute concentrations have made the quantitative determination of particulate absorption a…

Quantifying absorption by aquatic particles: A multiple scattering correction for glass-fiber filters

Abstract : Absorption spectra measured for aquatic particles concentrated onto glass-fiber filters require a correction for the increase in pathlength caused by multiple scattering in the glass-fiber…

Modeling in situ phytoplankton absorption from total absorption spectra in productive inland marine waters

Dissolved and suspended materials in the ocean modify the in‐water light field by absorbing and scattering photons. Direct measurement of inherent optical properties of individual optical…

A model for partitioning particulate absorption into phytoplanktonic and detrital components

Absorption and scattering coefficients in Irondequoit Bay1

Profiles of scalar, downwelling, upwelling, and spectral irradiance were measured on 37 dates in 1982-1983. Values of the attenuation coefficient K were 0.8-1.2 m-l, and the reflectance R was…

Optical efficiency factors of some phytoplankters1

Absorption and total scattering coefficients of four phytoplankton species grown in batch cultures were measured simultaneously. Backscattering coefficients were obtained by using an integrating…

Use of glass fiber filters for the rapid preparation of in vivo absorption spectra of photosynthetic bacteria

The preparation of in vivo absorption spectra of photosynthetic bacteria representing all four bacteriochlorophyll types is possible in a time of 3 min per sample, and the method proved to be useful in measurements of visible light absorption by particulate matter in the oceans.

Absorption of light by turbid materials

The transmission and absorption properties of turbid media have been examined with Kubelka and Munk’s theory of the optics of intensely scattering material. The equation for the optical density of…

Spectral absorption coefficients of living phytoplankton and nonalgal biogenous matter: A comparison between the Peru upwelling areaand the Sargasso Sea

Absorption coefficients of total particulate (biogenous) matter were determined in the mesotrophic waters of the Peruvian upwelling and the oligotrophic waters of the Sargasso Sea. A method based on…

A simple method to preserve oceanic phytoplankton for flow cytometric analyses.

A simple method was developed to preserve marine phytoplankton populations so that delayed flow cytometric analyses could be performed and preserved well both forward angle light scatter and chlorophyll fluorescence, but phycoerythrin fluorescence sometimes was significantly increased.