A new method for isolating primary mesenchyme cells of the sea urchin embryo. Panning on wheat germ agglutinin-coated dishes.

@article{Ettensohn1987ANM,
  title={A new method for isolating primary mesenchyme cells of the sea urchin embryo. Panning on wheat germ agglutinin-coated dishes.},
  author={Charles A Ettensohn and D R Mcclay},
  journal={Experimental cell research},
  year={1987},
  volume={168 2},
  pages={431-8}
}
This paper describes a rapid and efficient way to isolate primary mesenchyme cells (PMCs) of the sea urchin embryo. The procedure involves three simple steps: Dissociation of mesenchyme blastulae in calcium-free artificial seawater. Incubation of the resulting cell suspension on dishes that have been coated with wheat germ agglutinin (WGA), to which the PMCs adhere more firmly than do other cell types. Gentle rinsing of the dishes to remove loosely attached cells, followed by more vigorous… CONTINUE READING