A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair

@inproceedings{Britton2013ANM,
  title={A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair},
  author={S{\'e}bastien Britton and Julia A Coates and Stephen P. Jackson},
  booktitle={The Journal of cell biology},
  year={2013}
}
DNA double-strand breaks (DSBs) are the most toxic of all genomic insults, and pathways dealing with their signaling and repair are crucial to prevent cancer and for immune system development. Despite intense investigations, our knowledge of these pathways has been technically limited by our inability to detect the main repair factors at DSBs in cells. In this paper, we present an original method that involves a combination of ribonuclease- and detergent-based preextraction with high-resolution… CONTINUE READING