A new method for direct detection of the sites of actin polymerization in intact cells and its application to differentiated vascular smooth muscle.

Abstract

Here we report and validate a new method, suitable broadly, for use in differentiated cells and tissues, for the direct visualization of actin polymerization under physiological conditions. We have designed and tested different versions of fluorescently labeled actin, reversibly attached to the protein transduction tag TAT, and have introduced this novel… (More)
DOI: 10.1152/ajpcell.00210.2010

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