A new method based on cobalt for histochemical and cytochemical demonstration of glucose-6-phosphatase activity.

@article{Thiry1990ANM,
  title={A new method based on cobalt for histochemical and cytochemical demonstration of glucose-6-phosphatase activity.},
  author={Georges Thi{\'e}ry and J M Bernier and Michel Bergeron},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1990},
  volume={38 10},
  pages={1503-9}
}
We describe a novel technique for the histochemical and cytochemical demonstration of glucose-6-phosphatase activity. In this method, lead is replaced by cobalt. After activity of glucose-6-phosphatase, cobalt phosphate Co3(PO4)2 is formed, and in the presence of ammonium sulfide (NH4)2S, the precipitate is transformed into a sulfide that fixes osmium and provides good electron density. Glucose-6-phosphatase activity was determined mostly in rat kidney cells, but controls were also performed in… CONTINUE READING