A new member of the short-chain dehydrogenases/reductases superfamily: purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis.

@article{Ye2009ANM,
  title={A new member of the short-chain dehydrogenases/reductases superfamily: purification, characterization and substrate specificity of a recombinant carbonyl reductase from Pichia stipitis.},
  author={Qifa Ye and Ming Yan and Zhong Ping Yao and Lin Xu and Hou Cao and Zhengjiang Li and Yong Chen and Shuya Li and Jianxin Bai and Jian Xiong and Hanjie Ying and Pingkai Ouyang},
  journal={Bioresource technology},
  year={2009},
  volume={100 23},
  pages={6022-7}
}
A novel short-chain dehydrogenases/reductases superfamily (SDRs) reductase (PsCR) from Pichia stipitis that produced ethyl (S)-4-chloro-3-hydroxybutanoate with greater than 99% enantiomeric excess, was purified to homogeneity using fractional ammonium sulfate precipitation followed by DEAE-Sepharose chromatography. The enzyme purified from recombinant Escherichia coli had a molecular mass of about 35 kDa on SDS-PAGE and only required NADPH as an electron donor. The K(m) value of PsCR for ethyl… CONTINUE READING

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