A new mechanism for coactivation of transcription initiation: repositioning of an activator triggered by the binding of a second activator.

@article{Richet1991ANM,
  title={A new mechanism for coactivation of transcription initiation: repositioning of an activator triggered by the binding of a second activator.},
  author={Evelyne Richet and Dominique Vidal-Ingigliardi and Olivier Raibaud},
  journal={Cell},
  year={1991},
  volume={66 6},
  pages={1185-95}
}
The cAMP receptor protein (CRP) and MaIT, the maltose regulon activator, synergistically activate transcription from the E. coli maIKp promoter. The maIKp regulatory region comprises two series of MaIT-binding sites separated by three CRP-binding sites. By combining genetic and biochemical studies, we demonstrate that the promoter-proximal region contains two overlapping sets of three MaIT-binding sites. Occupation of the higher affinity set of sites, which occurs in the absence of CRP, does… CONTINUE READING