A new in vitro system for evaluation of passive intestinal drug absorption: establishment of a double artificial membrane permeation assay.

@article{Kataoka2014ANI,
  title={A new in vitro system for evaluation of passive intestinal drug absorption: establishment of a double artificial membrane permeation assay.},
  author={Makoto Kataoka and Saki Tsuneishi and Yukako Maeda and Yoshie Masaoka and Shinji Sakuma and Shinji Yamashita},
  journal={European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V},
  year={2014},
  volume={88 3},
  pages={840-6}
}
The aim of this present study was to establish a new in vitro assay, double artificial membrane permeation assay (DAMPA), to evaluate the human intestinal permeability of drugs. A double artificial membrane with an intracellular compartment was constructed in side-by-side chambers by sandwiching a filter containing buffer solution with impregnated lipophilic filters with dodecane containing 2w/v% phosphatidylcholine. Permeation data of ionic compounds clearly indicated that not only the pH… CONTINUE READING