A new immunization procedure for the obtention of anti-leucine enkephalin antibodies: Part I. Immunization procedure and physicochemical characteristics of antibodies

@article{Cupo1986ANI,
  title={A new immunization procedure for the obtention of anti-leucine enkephalin antibodies: Part I. Immunization procedure and physicochemical characteristics of antibodies},
  author={Annie Cupo and Jean Vion-Dury and Th. Jarry},
  journal={Neuropeptides},
  year={1986},
  volume={8},
  pages={207-212}
}
We described here a new immunization procedure to obtain high titre and high specific antibodies against Leu-enkephalin (LE). The immunogen form is composed of one part of LE conjugate and one part of LE-Arg6 conjugate. We have observed an increase of titre, affinity and specificity of the antibodies in the coimmunization procedure compared to those obtained by conventional immunization involving only the LE conjugate. The Leu-enkephalin antibodies exhibit a high affinity (KD 8 X 10(-12) M) and… CONTINUE READING

Topics from this paper.