A new immobilization and sensing platform for nitrate quantification.

  title={A new immobilization and sensing platform for nitrate quantification.},
  author={Veena Sachdeva and Vinita Hooda},
  • Veena Sachdeva, Vinita Hooda
  • Published 2014
  • Chemistry, Medicine
  • Talanta
  • Nitrate reductase (NR) from Aspergillus niger was covalently coupled to the epoxy affixed gold nanoparticles (epoxy/AuNPs) with a conjugation yield of 35.40±0.01 μg/cm(2) and 93.90±0.85% retention of specific activity. The bare and NR bound epoxy/AuNPs support was characterized using scanning electron microscopy and Fourier Transform Infrared Spectroscopy. The immobilized enzyme system was optimized with respect to pH, temperature and substrate concentrations and successfully employed for… CONTINUE READING
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