A new human ovarian carcinoma cell line: establishment and analysis of tumor-associated markers.

Abstract

In the present study we describe the establishment and characteristics of a new human tumor cell line (OV-1063) positive for carcinoembryonic antigen (CEA) originating from ovarian metastatic tumor cells. Analysis of the cultured cells during their in vitro adaptation period revealed while the primary culture exhibited a low proportion of CEA-positive cells, this proportion increased with culture passages and eventually more than 90% of the cells in the established line were CEA-positive. Thus, during the period of adaptation to in vitro growth, a selection for CEA-positive cells took place but the amount of CEA secreted per each positive cell seemed to be constant. Several tumor-associated characteristics were found positive on the established OV-1063 cell line. The in vitro growing cell line exhibited an abnormal chromosome pattern with a near-trisomy karyotype for some chromosomes, colony formation in soft agar as well as positive staining with a monoclonal antibody B38.1. Culture supernatants of the OV-1063 cells contained significant amounts of CEA as well as CA-125 antigen which is an ovarian-carcinoma-associated antigen.

Cite this paper

@article{Horowitz1985ANH, title={A new human ovarian carcinoma cell line: establishment and analysis of tumor-associated markers.}, author={Aviva T. Horowitz and Abraham J. Treves and R. Vo\ss and Elena B. Okon and Zvi Y Fuks and Lee Davidson and Shoshana Biran}, journal={Oncology}, year={1985}, volume={42 5}, pages={332-7} }