A new histochemical method for the identification and visualization of both side chain acylated and nonacylated sialic acids.

@article{Culling1976ANH,
  title={A new histochemical method for the identification and visualization of both side chain acylated and nonacylated sialic acids.},
  author={C. F. A. Culling and Philip E. Reid and William L. Dunn},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1976},
  volume={24 12},
  pages={1225-30}
}
A new histochemical method is described for the differentiation of mucins that utilizes two different Schiff reagents and allows single section identification of side chain O-acylated, and nonacylated, sialic acids in contrasting colors. In the event of mucins containing only one type of sialic acid, it may allow their specific identification (e.g., C7 or C8 side chain O-acylated). It has been shown to be useful in the identification of some metastases from adenocarcinomas of colon (where the… CONTINUE READING