A new high-performance thin layer chromatography-based assay of detergents and surfactants commonly used in membrane protein studies.

@article{Barret2013ANH,
  title={A new high-performance thin layer chromatography-based assay of detergents and surfactants commonly used in membrane protein studies.},
  author={Laurie-Anne Barret and Ange Polidori and Françoise Bonnet{\'e} and Pierre Bernard-Savary and Colette Jungas},
  journal={Journal of chromatography. A},
  year={2013},
  volume={1281},
  pages={135-41}
}
The hydrophobic nature of membrane proteins (MPs) necessitates the use of detergents for their extraction, solubilization and purification. Because the concentration of amphiphiles is crucial in the crystallization process, detergent quantification is essential to routine analysis. Here we describe a quantitative high-performance thin-layer chromatography (HPTLC) method we developed for the detection of small quantities of detergent bound to solubilized MPs. After optimization of aqueous… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-5 of 5 references

Nat

  • R. M. Bill, P. J. Henderson, +7 authors H. Vogel
  • Biotechnol. 29
  • 2011
1 Excerpt

Chem

  • L. Li, S. Nachtergaele, +4 authors J. Am
  • Soc. 130
  • 2008
1 Excerpt

E

  • H. Michel, M. Rossman
  • Arnold (Eds.), International Tables for…
  • 2001
1 Excerpt

Curr

  • C. Ostermeier, H. Michel
  • Opin. Struct. Biol. 7
  • 1997
1 Excerpt

Biomembr

  • R. M. Garavito, D. Picot, P. J. Loll, J. Bioenerg
  • 28
  • 1996
1 Excerpt

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