A new-generation stable inducible packaging cell line for lentiviral vectors.

@article{Farson2001ANS,
  title={A new-generation stable inducible packaging cell line for lentiviral vectors.},
  author={Deborah A. Farson and R. Witt and R. P. McGuinness and Terry L Dull and Mike Kelly and Jae-Jin Song and Rico Radeke and Anatoly Bukovsky and A. Consiglio and Luigi Naldini},
  journal={Human gene therapy},
  year={2001},
  volume={12 8},
  pages={981-97}
}
We have successfully generated and characterized a stable packaging cell line for HIV-1-based vectors. To allow safe production of vector, a minimal packaging construct carrying only the coding sequences of the HIV-1 gag-pol, tat, and rev genes was stably introduced into 293G cells under the control of a Tet(o) minimal promoter. 293G cells express the chimeric Tet(R)/VP16 trans-activator and contain a tetracycline-regulated vesicular stomatitis virus protein G (VSV-G) envelope gene. When the… CONTINUE READING
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