A new filtering technique for removing anti-Stokes emission background in gated CW-STED microscopy.

@article{Hernndez2014ANF,
  title={A new filtering technique for removing anti-Stokes emission background in gated CW-STED microscopy.},
  author={I Coto Hern{\'a}ndez and Chiara Peres and Francesca Cella Zanacchi and Marta d'Amora and Sotirios Christodoulou and Paolo Bianchini and Alberto Diaspro and Giuseppe Vicidomini},
  journal={Journal of biophotonics},
  year={2014},
  volume={7 6},
  pages={376-80}
}
Stimulated emission depletion (STED) microscopy is a prominent approach of super-resolution optical microscopy, which allows cellular imaging with so far unprecedented unlimited spatial resolution. The introduction of time-gated detection in STED microscopy significantly reduces the (instantaneous) intensity required to obtain sub-diffraction spatial resolution. If the time-gating is combined with a STED beam operating in continuous wave (CW), a cheap and low labour demand implementation is… CONTINUE READING