A new facile method for spin-labeling of oligonucleotides.

@article{Strube2001ANF,
  title={A new facile method for spin-labeling of oligonucleotides.},
  author={T Strube and Olav Schiemann and Fraser Macmillan and Thomas F Prisner and Joachim W. Engels},
  journal={Nucleosides, nucleotides & nucleic acids},
  year={2001},
  volume={20 4-7},
  pages={1271-4}
}
A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.