A new expression vector for Crithidia fasciculata and Leishmania.

  title={A new expression vector for Crithidia fasciculata and Leishmania.},
  author={Emmanuel T{\'e}taud and Isabelle Lecuix and Tara A. Sheldrake and Th{\'e}o Baltz and Alan H. Fairlamb},
  journal={Molecular and biochemical parasitology},
  volume={120 2},
Crithidia fasciculata is a monogenetic parasite of insects. It grows in fully defined media without requiring serum, which facilitates biochemical analysis. We have constructed a series of expression systems that allows expression of transfected genes in the kinetoplastid protozoa Crithidia and Leishmania. These cells can be readily transfected with plasmid DNA by electroporation and transformants selected with various antibiotic resistance markers. 5'-Trans-splicing signals and poorly defined… CONTINUE READING


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