A new enzyme immunoassay to detect antibodies to arboviruses in the blood of wild birds.

@article{Chiles1998ANE,
  title={A new enzyme immunoassay to detect antibodies to arboviruses in the blood of wild birds.},
  author={Robert E. Chiles and William K. Reisen},
  journal={Journal of vector ecology : journal of the Society for Vector Ecology},
  year={1998},
  volume={23 2},
  pages={123-35}
}
A new indirect enzyme immunoassay (EIA) was developed to screen wild bird sera for antibodies against western equine encephalomyelitis (WEE) and St. Louis encephalitis (SLE) viruses. The detector antibody was made by immunizing rabbits with serum proteins pooled from single species representatives of four bird orders and was conjugated with horseradish peroxidase to allow visualization with the ABTS substrate in an EIA plate reader set at 405 nm. The detector antibody recognized a wide range of… CONTINUE READING

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