A new enzyme immunoassay for aconitine and its application to quantitative determination of aconitine levels in plasma.

@article{Tazawa2003ANE,
  title={A new enzyme immunoassay for aconitine and its application to quantitative determination of aconitine levels in plasma.},
  author={Takako Tazawa and Huai-qing Zhao and Yan Li and Meselhy Ragab Meselhy and Norio Nakamura and Teruaki Akao and Masao Hattori},
  journal={Biological & pharmaceutical bulletin},
  year={2003},
  volume={26 9},
  pages={1289-94}
}
A reliable enzyme immunoassay (EIA) method was developed for quantitative determination of aconitine with high sensitivity and specificity. The bovine serum albumin (BSA)- and beta-galactosidase (beta-Gal) conjugates as immunogens and enzyme-labeled antigens were prepared by coupling of their proteins with succinic acid (short chain length; n=2, where n represents the number of methylene units) and hexadecanedioic acid (long chain length; n=14) hemiesters of benzoylaconine through the… CONTINUE READING