A new enzymatic cycling technique for glutamate determination in brain microdialysates.

@article{Mitani1990ANE,
  title={A new enzymatic cycling technique for glutamate determination in brain microdialysates.},
  author={Akihisa Mitani and Hirohiko Kubo and Katsumi Iga and H Imon and F Kadoya and Kouhei Kataoka},
  journal={Journal of neurochemistry},
  year={1990},
  volume={54 2},
  pages={709-11}
}
A quantitative analysis of glutamate in brain dialysate was made by using an enzymatic cycling technique. This method made it possible to measure the concentration of glutamate in dialysate collected at 30-s intervals. Dialysates were collected from Mongolian gerbil hippocampus before, during, and after two 90-s ischemic insults at an interval of 5 min. An extracellular increase in levels of glutamate was already observed in samples collected during a 30-60 s period after the onset of each… CONTINUE READING

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