A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode.

@article{Steen2002AND,
  title={A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode.},
  author={Hanno Steen and Matthias Mann},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2002},
  volume={13 8},
  pages={996-1003}
}
Although numerous strategies have been devised to analyze protein phosphorylation, an abundant intracellular protein modification, there is still a need for different methods for the analysis of this modification. A method to both detect and localize the phosphorylation within a protein/peptide is especially required. In this paper, a new strategy is described, which makes use of beta-elimination/Michael addition reactions to introduce a functional group at the original site of phosphorylation… CONTINUE READING