A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry.

@article{Huo2012AND,
  title={A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry.},
  author={Feifeng Huo and Xin Wang and Yehua Han and Yu Bai and Wei Zhang and Hancheng Yuan and Huwei Liu},
  journal={Talanta},
  year={2012},
  volume={99},
  pages={
          420-5
        }
}
In this work, a new labeling reagent, 2-bromopyridine-5-boronic acid (BPBA), was introduced to derivatize brassinosteroids (BRs). The BPBA not only provided a very simple and rapid labeling procedure, but also remarkably increased the detection sensitivity of BRs. Based on this new labeling reaction, a rapid and sensitive method for BRs' analysis in Arabidopsis thaliana was established by using the ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass… CONTINUE READING

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