A new cytolethal distending toxin (CDT) from Escherichia coli producing CNF2 blocks HeLa cell division in G2/M phase

@article{Peres1997ANC,
  title={A new cytolethal distending toxin (CDT) from Escherichia coli producing CNF2 blocks HeLa cell division in G2/M phase},
  author={S. Pérès and O. Marchés and F. Daigle and J. Nougayr{\`e}de and F. Hérault and C. Tasca and J. De Rycke and {\'E}. Oswald},
  journal={Molecular Microbiology},
  year={1997},
  volume={24}
}
Escherichia coli strain 1404, isolated from a septicaemic calf, carries a transferable plasmid called pVir which codes for the cytotoxic necrotizing factor type 2 (CNF2). A 4 h interaction between strain 1404 and HeLa cells induced the formation of giant mononucleated cells blocked in G2/M phase. Mating experiments between strain 1404 and a non‐pathogenic recipient strain demonstrated that the factor(s) encoded by pVir mediated the cell‐cycle arrest. A 3.3 kb DNA fragment isolated from a DNA… Expand
The cytolethal distending toxin-IV cdt coding region in an avian pathogenic Escherichia coli (APEC) strain shows instability and irregular excision pattern.
TLDR
The failure of in vitro transfer of cdt genes into a porcine EPEC E. coli strain suggests that the deletion ofcdt-IV flanking genes alone do not promote the spread of cdT-IV, and suggests the presence of an unstable hitherto unknown genomic region. Expand
Cytolethal Distending Toxin Type I and Type IV Genes Are Framed with Lambdoid Prophage Genes in Extraintestinal Pathogenic Escherichia coli
TLDR
The results suggest that the cdt-I andcdt-IV genes might have been acquired from a common ancestor by phage transduction and evolved in their bacterial hosts and play a crucial role in the generation of genetic diversity within ExPEC. Expand
An inducible lambdoid prophage encoding cytolethal distending toxin (Cdt-I) and a type III effector protein in enteropathogenic Escherichia coli
TLDR
The results suggest that CDT-1Φ is the latest member of a growing family of lambdoid phages encoding bacterial cyclomodulins and that the phage may have a role in horizontal transfer of these virulence genes. Expand
Cytolethal distending toxin A, B and C subunit proteins are necessary for the genotoxic effect of Escherichia coli CDT-V.
TLDR
It is shown that all the three CDT subunit proteins are necessary for the genotoxic effect caused by CDT-V, and expression clones containing the cdt-V subunit genes were constructed. Expand
Sequence Variability of P2-Like Prophage Genomes Carrying the Cytolethal Distending Toxin V Operon in Escherichia coli O157
TLDR
The results suggest that P2-like phages play a role in the dissemination of cdt-V between E. coli O157 strains and that after integration into the bacterial chromosome, they adapted to the respective hosts and became temperate. Expand
Sequence Variability of P 2-Like Prophage Genomes Carrying the Cytolethal Distending Toxin V Operon in Escherichia coli O 157
Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far.Expand
Cytolethal distending toxin (CDT): a bacterial weapon to control host cell proliferation?
TLDR
The putative nuclease activity of CDT-B, together with the activation by CDT of a G2 cell cycle checkpoint, strongly suggests that CDT induces an as yet uncharacterized DNA alteration. Expand
Molecular profile and genetic diversity of cytolethal distending toxin (CDT)‐producing Escherichia coli isolates from diarrheal patients
  • M. Oloomi, S. Bouzari
  • Biology, Medicine
  • APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
  • 2008
TLDR
There is no common pattern of insertion among these isolates although they do share a common trait of CDT production, and observed diversity could be an independent acquisition of virulence genes that might occur through horizontal gene transfer by mobile genetic elements. Expand
Cytolethal Distending Toxin from Shiga Toxin-Producing Escherichia coli O157 Causes Irreversible G2/M Arrest, Inhibition of Proliferation, and Death of Human Endothelial Cells
TLDR
It is demonstrated that CDT-V causes irreversible damage to human endothelial cells and thus may contribute to the pathogenesis of STEC-mediated diseases. Expand
Molecular Characterizations of Cytolethal Distending Toxin Produced by Providencia alcalifaciens Strains Isolated from Patients with Diarrhea
TLDR
This is the first report showing that certain clinical P. alcalifaciens strains could produce variants of the CDTs compared, and Interestingly, the CDT of P.AlcalIFaciens (PaCDT) showed some homology with theCDT of Shigella boydii. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 73 REFERENCES
Cloning and sequencing of the genes encoding Escherichia coli cytolethal distending toxin
Escherichia coli strains expressing cytolethal distending toxin (CDT) cause elongation of CHO cells at 24 h, followed by progressive cellular distention and death for up to 120 h. Similar distentionExpand
Gene block encoding production of cytotoxic necrotizing factor 1 and hemolysin in Escherichia coli isolates from extraintestinal infections
TLDR
DNA hybridization experiments indicated that the CNF1 and hemolysin determinants were closely linked on the chromosomes of isolate E-B35 and six additional extraintestinal isolates belonging to serogroups O2, O4, O6, O22, O75, and O85. Expand
Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene
TLDR
PCR experiments indicated the probable presence of cdtB sequences in all of these Campylobacter species, although the HeLa cell assay indicated that most C. jejuni strains produce significantly higher CDT titers than do C. coli strains. Expand
Cloning, sequencing, and expression of the Escherichia coli cytolethal distending toxin genes
TLDR
DNA was isolated from the CDT-producing E. coli strain, 9142-88, and cloned into a cosmid vector and a plasmid with a 4-kb insert which still encoded the toxin activity was obtained, suggesting the products of all three open reading frames may be required for toxin activity. Expand
A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli isolates from clinical material.
TLDR
A new heat-labile Escherichia coli toxin cytolethal to Vero, HeLa, HEp-2 and CHO cells and negative in Y-1 cells has been demonstrated in culture filtrates of 43 E. coli strains associated with diarrheal disease and demonstrated a molecular weight over 30,000, was nondialyzable and trypsin-sensitive. Expand
A new heat-labile cytolethal distending toxin (CLDT) produced by Campylobacter spp.
TLDR
The new CLDT toxin could only be neutralized by homologous rabbit antitoxin, was trypsin-sensitive, nondialyzable and over 30,000 in molecular weight. Expand
Mitotic block and delayed lethality in HeLa epithelial cells exposed to Escherichia coli BM2-1 producing cytotoxic necrotizing factor type 1
TLDR
The results suggest that a gene downstream of cnf-1, essential to the induction of the CPE, was affected by the mutation, and transformation of the wild-type strain BM2-1 with the same recombinant plasmid leads to a significant increase in both CNF1 activity and CPE. Expand
Examination of diarrheagenicity of cytolethal distending toxin: suckling mouse response to the products of the cdtABC genes of Shigella dysenteriae
TLDR
The results indicate that CDT is diarrheagenic to suckling mice and suggest that diarrheaheagenicity is dependent on CDT level, while preparations containing wild-type CDT induced tissue damage (necrosis and reparative hyperplasia) in the descending colon, whereas the tissues of the small intestines remained apparently intact. Expand
Response of Chinese hamster ovary cells to a cytolethal distending toxin (CDT) of Escherichia coli and possible misinterpretation as heat-labile (LT) enterotoxin
TLDR
Increased incubation of the Chinese hamster ovary cell (CHO) assay allowed differentiation of cytotonic and cytotoxic effects and continued incubation for 96–120 h was essential for observation of progressive morphological changes and cytolethal events. Expand
Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers.
TLDR
It is proposed that Rho proteins are the targets of CNF2 in mammalian cells as well as the formation of stress fibers in eukaryotic cells with a Rho-specific ADP-ribosyltransferase. Expand
...
1
2
3
4
5
...