A new colorimetric microtitre model for the detection of Staphylococcus aureus biofilms.

@article{Tot2008ANC,
  title={A new colorimetric microtitre model for the detection of Staphylococcus aureus biofilms.},
  author={Kim Tot{\'e} and Dirk A Vanden Berghe and Louis Maes and P. Merino de Cos},
  journal={Letters in applied microbiology},
  year={2008},
  volume={46 2},
  pages={249-54}
}
AIMS Research on biofilms requires validated quantitative models that focus both on matrix and viable bacterial mass. In this study, a new microplate model for the detection of Staphylococcus aureus biofilms was developed. METHODS AND RESULTS Dimethyl methylene blue (DMMB) dye was used to quantify biofilm matrix colorimetrically. Initially developed for the detection of glycosaminoglycans, the DMMB protocol was optimized for S. aureus biofilm research. In addition, the redox indicator… CONTINUE READING

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