A new class of homogeneous nucleic acid probes based on specific displacement hybridization.

@article{Li2002ANC,
  title={A new class of homogeneous nucleic acid probes based on specific displacement hybridization.},
  author={Qingge Li and Guoyan Luan and Qiuping P Guo and Jixuan Liang},
  journal={Nucleic acids research},
  year={2002},
  volume={30 2},
  pages={E5}
}
We have developed a new class of probes for homogeneous nucleic acid detection based on the proposed displacement hybridization. Our probes consist of two complementary oligodeoxyribonucleotides of different length labeled with a fluorophore and a quencher in close proximity in the duplex. The probes on their own are quenched, but they become fluorescent upon displacement hybridization with the target. These probes display complete discrimination between a perfectly matched target and single… CONTINUE READING

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