A new carbene based heterobifunctional reagent. Photochemical crosslinking of aldolase.

@article{Mogre1987ANC,
  title={A new carbene based heterobifunctional reagent. Photochemical crosslinking of aldolase.},
  author={R M Mogre and H F Batliwala and P S Anjaneyulu and Anil K. Lala},
  journal={FEBS letters},
  year={1987},
  volume={221 2},
  pages={408-14}
}
The synthesis of a new photoactivatable heterobifunctional crosslinking reagent, the N-oxysuccinimide ester of 2-carboxy-9-diazofluorene, is described. The ability of the parent chromophore 2-carbomethoxy-9-diazofluorene to insert into cyclohexane and methanol has been established. The reagent has been linked to aldolase and the stoichiometry determined… CONTINUE READING