A new blood–brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes

  title={A new blood–brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes},
  author={Shinsuke Nakagawa and M{\'a}ria A Deli and Hiroko Kawaguchi and Takeshi Shimizudani and Masami Niwa},
  journal={Neurochemistry International},
Blood-brain barrier (BBB) characteristics are induced and maintained by cross-talk between brain microvessel endothelial cells and neighbouring elements of the neurovascular unit. While pericytes are the cells situated closest to brain endothelial cells morphologically and share a common basement membrane, they have not been used in co-culture BBB models for testing drug permeability. We have developed and characterized a new syngeneic BBB model using primary cultures of the three main cell… CONTINUE READING
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Publications referenced by this paper.
Showing 1-10 of 48 references

Blood – brain barrier models

M. A. Deli
View 6 Excerpts
Highly Influenced

Blood-brain barrier models. In: Lajtha, A. (Ed.) Handbook of Neurochemistry and Molecular Neurobiology

M. A. Deli
Neural Membranes and Transport • 2007
View 7 Excerpts
Highly Influenced

The critical component to establish in vitro BBB model: Pericyte.

Brain research. Brain research reviews • 2005
View 7 Excerpts
Highly Influenced

Hydrocortisone reinforces the blood-brain barrier properties in a serum free cell culture system.

Biochemical and biophysical research communications • 1998
View 5 Excerpts
Highly Influenced

Modelling of the blood–brain barrier in drug discovery and development

Nature Reviews Drug Discovery • 2007
View 8 Excerpts
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