A new bioartificial liver using porcine hepatocyte spheroids in high-cell-density suspension perfusion culture: in vitro performance in synthesized culture medium and in 100% human plasma.

Abstract

A prototype of a bioartificial liver (BAL) based on suspension perfusion culture of porcine hepatocyte spheroids was developed at 150 ml scale. About 2% (4 x 10(9) cells) of whole human liver cells was immobilized. The cell density in the bioreactor was 2.7 x 10(7) cells/ml, which was almost comparable to that of presently developed packed-bed-type BALs… (More)

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@article{Sakai1999ANB, title={A new bioartificial liver using porcine hepatocyte spheroids in high-cell-density suspension perfusion culture: in vitro performance in synthesized culture medium and in 100% human plasma.}, author={Yasuyuki Sakai and Kiyoko Naruse and Ikuo Nagashima and Tetsuichiro Muto and M Suzuki}, journal={Cell transplantation}, year={1999}, volume={8 5}, pages={531-41} }