A new assay for tRNA aminoacylation kinetics.

  title={A new assay for tRNA aminoacylation kinetics.},
  author={Alexey D Wolfson and Jeffrey A Pleiss and O. C. Uhlenbeck},
  volume={4 8},
An improved quantitative assay for tRNA aminoacylation is presented based on charging of a nicked tRNA followed by separation of an aminoacylated 3'-fragment on an acidic denaturing polyacrylamide gel. Kinetic parameters of tRNA aminoacylation by Escherichia coli AlaRS obtained by the new method are in excellent agreement with those measured by the conventional method. This assay provides several advantages over the traditional methods of measuring tRNA aminoacylation: (1) the fraction of… CONTINUE READING


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