A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration.

@article{Koebe1990ANA,
  title={A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration.},
  author={Hans G. Koebe and Jay C Dunn and Mehmet Toner and Laura M Sterling and Allison Hubel and Ernest G. Cravalho and Martin L. Yarmush and Ronald G. Tompkins},
  journal={Cryobiology},
  year={1990},
  volume={27 5},
  pages={576-84}
}
Current methods of cryopreservation of hepatocytes in single cell suspensions result in low overall yields of hepatocytes, demonstrating long-term preservation of hepatocellular functions. A novel culture method has recently been developed to culture liver cells in a sandwich configuration of collagen layers in order to stabilize the phenotypic expression of these cells in vitro (J. C. Y. Dunn, M. L. Yarmush, H. G. Koebe, and R. G. Tompkins, FASEB J. 3, 174, 1989). Using this culture system… CONTINUE READING