Durchffihrung und Auswertung enteraler sowie parenteraler akuter Toxizit~its
- L Cavalli-Sforza
170 acute toxicity determinations, carried out in rats, mice, and guinea pigs after oral and parenteral administration during the past 5 years, using 5 ♂/5 ♀ animals per dose, were evaluated with respect to the possibility of a reduction of animals necessary for obtaining LD50 values with limits of confidence. Calculations were performed on existing data; no additional animal experiments were initiated for the purpose of this paper. For the majority of substances 3 ♂/3 ♀ animals per dose would have sufficed for the determination of a LD50 value with limits of confidence. Even when using 2 ♂/2 ♀ animals per dose, in most cases sufficently acceptable LD50 values can be determined, if the period of observation — conventionally 4 weeks — would be doubled. Using this procedure, 75% of the animals could be saved. The reduced number of animals and the labor saved thereby would allow for a more individualized observation of single animals thus optimizing the evaluation of acute toxicity testing.