A new approach to an influenza live vaccine: modification of the cleavage site of hemagglutinin

@article{Stech2005ANA,
  title={A new approach to an influenza live vaccine: modification of the cleavage site of hemagglutinin},
  author={J{\"u}rgen Stech and Holger Garn and Mich{\`e}le Wegmann and Ralf Wagner and H D Klenk},
  journal={Nature Medicine},
  year={2005},
  volume={11},
  pages={683-689}
}
A promising approach to reduce the impact of influenza is the use of an attenuated, live virus as a vaccine. Using reverse genetics, we generated a mutant of strain A/WSN/33 with a modified cleavage site within its hemagglutinin, which depends on proteolytic activation by elastase. Unlike the wild-type, which requires trypsin, this mutant is strictly dependent on elastase. Both viruses grow equally well in cell culture. In contrast to the lethal wild-type virus, the mutant is entirely… CONTINUE READING