A new approach for the biolistic method: Bombardment of living nitrogen-fixing bacteria into plant tissues

Abstract

A new utilization of the biolistic gun was developed for the direct introduction of nitrogen-fixing bacteria (Azotobacter vinelandii) into strawberry (Fragaria x ananassa) tissues. This was the first case of using living bacteria as microprojectiles for the bombardment of plant tissues. Bacterial cells, adhered to tungsten particles, were accelerated by a… (More)
DOI: 10.1079/IVP2003444

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