A new application of BCG antibody for rapid screening of various tissue microorganisms.

Abstract

BACKGROUND In routine dermatopathology there is growing demand for a simple, fast, cost-effective, and highly sensitive screening tool for the detection of microorganisms. OBJECTIVE Our purpose was to determine whether immunostaining with polyclonal anti-Mycobacterium bovis (BCG), which is known for its interspecies cross-reactivity, is a suitable screening method for many common microorganisms in dermatopathologic specimens. METHODS A total of 254 formalin-fixed, paraffin-embedded skin specimens of viral, protozoal, fungal, and bacterial infections were stained with appropriate histochemical stains and with anti-BCG. RESULTS Anti-BCG labeled bacteria and fungi with high sensitivity and minimal background staining, but did not react with spirochetes, viruses, or protozoa (Leishmania). The quality and sensitivity of anti-BCG staining were superior to conventional histochemical stains. CONCLUSION Because of its cross-reactivity with many bacteria and fungi as well as its high sensitivity and minimal background staining, the anti-BCG immunostain is a promising screening tool for the detection of the most common bacterial and fungal microorganisms in paraffin-embedded skin specimens.

Statistics

0204060'03'05'07'09'11'13'15'17
Citations per Year

183 Citations

Semantic Scholar estimates that this publication has 183 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Kutzner1998ANA, title={A new application of BCG antibody for rapid screening of various tissue microorganisms.}, author={Heinz Kutzner and Zsolt B. Argenyi and Luis Requena and Arno Ruetten and Harald H{\"{u}gel}, journal={Journal of the American Academy of Dermatology}, year={1998}, volume={38 1}, pages={56-60} }