A new apparatus and surgical technique for the dual perfusion of human tumor xenografts in situ in nude rats.

@article{Dauchy2012ANA,
  title={A new apparatus and surgical technique for the dual perfusion of human tumor xenografts in situ in nude rats.},
  author={Robert T. Dauchy and Erin M. Dauchy and Lulu Mao and Victoria P. Belancio and Steven Marc Hill and David E. Blask},
  journal={Comparative medicine},
  year={2012},
  volume={62 2},
  pages={99-108}
}
We present a new perfusion system and surgical technique for simultaneous perfusion of 2 tissue-isolated human cancer xenografts in nude rats by using donor blood that preserves a continuous flow. Adult, athymic nude rats (Hsd:RH-Foxn1(rnu)) were implanted with HeLa human cervical or HT29 colon adenocarcinomas and grown as tissue-isolated xenografts. When tumors reached an estimated weight of 5 to 6 g, rats were prepared for perfusion with donor blood and arteriovenous measurements. The… CONTINUE READING