A new and rapid colorimetric determination of acetylcholinesterase activity.

@article{Ellman1961ANA,
  title={A new and rapid colorimetric determination of acetylcholinesterase activity.},
  author={George L. Ellman and Kevin D. Courtney and V ANDRES and R M FEATHER-STONE},
  journal={Biochemical pharmacology},
  year={1961},
  volume={7},
  pages={88-95}
}
A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described. The enzyme activity is measured by following the increase of yellow color produced from thiocholine when it reacts with dithiobisnitrobenzoate ion. It is based on coupling of these reactions: (enzyme) acetylthiocholine -----+ thiocholine + acetate thiocholine + dithiobisnitrobenzoate -+ yellow color The latter reaction is rapid and the assay is… CONTINUE READING
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