A new Raman spectroscopic probe of both the protonation state and noncovalent interactions of histidine residues.


The amino acid histidine (His) has a number of unique roles that can dictate function in proteins, and these roles are typically conferred through noncovalent interactions that depend on the protonation state of His's 4-substituted imidazole ring. His's protonation state can vary near physiological pH, and a probe of His's variable protonation state and its… (More)
DOI: 10.1021/jp311815k