A new 5′ terminal murine GAPDH exon identified using 5′RACE LaNe

  title={A new 5′ terminal murine GAPDH exon identified using 5′RACE LaNe},
  author={Daniel J. Park},
  journal={Molecular Biotechnology},
In this work, a ligation-independent, fully gene-specific, nested polymerase chain reaction (PCR) method for the elucidation of 5′ cDNA sequence is described and demonstrated for the first time. Two manifestations of the method, rapid amplification of cDNA ends (RACE) by lariat-dependent nested PCR 5′ (RACE LaNe), at least as simple to perform as conventional RACE, were successfully applied to the murine housekeeping genes phosphoglycerate kinase 1 (PGK1), β-actin (β-ACT), and glyceraldehyde-3… 
6 Citations

Lariat-dependent nested PCR for flanking sequence determination.

Methods detailed in this chapter relate to the use of Lariat-dependent Nested (LaNe) PCR to characterize unknown RNA or DNA sequence flanking known regions. A multitude of approaches designed to


The polymerase chain reaction (PCR) is a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to

Complete genomic sequence of an infectious pancreatic necrosis virus isolated from rainbow trout (Oncorhynchus mykiss) in China

The sequence of ChRtm213 was the first gene sequence of IPNV isolates in China, and the results suggest that the Chinese IPNV isolate has relative closer relationship with Japanese IPNV strains.



3' RACE LaNe: a simple and rapid fully nested PCR method to determine 3'-terminal cDNA sequence.

3′ RACE LaNe is demonstrated: a simple and rapid fully nested PCR method to determine 3′-terminal cDNA sequence that outperforms a conventional heminested anchored PCR 3′ Race series when it is applied to the long and complex tammar wallaby ATRX gene and the mouse PGK1 and GAPD genes.

Ligation-mediated amplification of RNA from murine erythroid cells reveals a novel class of beta globin mRNA with an extended 5'- untranslated region

The evolutionary conservation of this relationship suggests the importance of the GATA promoter element of the mouse beta globin gene and its possible involvement in developmental regulation of expression of this gene.

A general method for cloning eukaryotic structural gene sequences.

The susceptibility of inserted beta globin, sequences to the restriction endonuclease EcoRI confirms the existence of a site already found through previous nucleotide sequence analysis, as well as estimating the amount of inserted globin sequences.

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

The efficacy of this cDNA cloning strategy was demonstrated by isolating cDNA clones of mRNA from int-2, a mouse gene that expresses four different transcripts at low abundance, the longest of which is approximately 2.9 kilobases.

Stepwise biosynthesis in vitro of globin genes from globin mRNA by DNA polymerase of avian myeloblastosis virus.

  • F. RougeonB. Mach
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1976
Data indicate that a loop structure on the 3' end of cDNA allowed DNA synthesis to take place by a "self-priming" mechanism, and some of the double-stranded DNA synthesized corresponded to the entire sequence of the 9S mRNA template.

New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase.

  • M. Sirover
  • Biology
    Biochimica et biophysica acta
  • 1999

A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.

A procedure that extends this technique to sequences that lie outside the boundaries of known sequences by inversion of the sequence of interest by circularizaton and re-opening at a different site is described.

High-efficiency full-length cDNA cloning by biotinylated CAP trapper.

We have devised a method for efficiently constructing high-content full-length cDNA libraries based on chemical introduction of a biotin group into the diol residue of the cap structure of eukaryotic