A new 5′ terminal murine GAPDH exon identified using 5′RACE LaNe

@article{Park2005AN5,
  title={A new 5′ terminal murine GAPDH exon identified using 5′RACE LaNe},
  author={D. Park},
  journal={Molecular Biotechnology},
  year={2005},
  volume={29},
  pages={39-46}
}
  • D. Park
  • Published 2005
  • Biology, Medicine
  • Molecular Biotechnology
  • In this work, a ligation-independent, fully gene-specific, nested polymerase chain reaction (PCR) method for the elucidation of 5′ cDNA sequence is described and demonstrated for the first time. Two manifestations of the method, rapid amplification of cDNA ends (RACE) by lariat-dependent nested PCR 5′ (RACE LaNe), at least as simple to perform as conventional RACE, were successfully applied to the murine housekeeping genes phosphoglycerate kinase 1 (PGK1), β-actin (β-ACT), and glyceraldehyde-3… CONTINUE READING

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