A network of orthogonal ribosome·mRNA pairs

  title={A network of orthogonal ribosome·mRNA pairs},
  author={Oliver Rackham and Jason W. Chin},
  journal={Nature Chemical Biology},
Synthetic biology promises the ability to program cells with new functions. Simple oscillators, switches, logic functions, cell-cell communication and pattern-forming circuits have been created by the connection of a small set of natural transcription factors and their binding sites in different ways to produce different networks of molecular interactions. However, the controlled synthesis of more complex synthetic networks and functions will require an expanded set of functional molecules with… 
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Controlling orthogonal ribosome subunit interactions enables evolution of new function
E engineered orthogonal ‘stapled’ ribosomes are discovered (with subunits linked through an optimized RNA staple) with activities comparable to that of the parent Orthogonal ribosome; they minimize association with endogenous subunits and mediate translation of orthosomal mRNAs through the association of stapled subunits.
A fully orthogonal system for protein synthesis in bacterial cells
The authors invert the Ribo-T system, using the engineered ribosomes to translate the proteome while the native ribosome translates specific mRNA.
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Ribo-T is used to create the first fully orthogonal ribosome–messenger RNA system, and demonstrate its evolvability by selecting otherwise dominantly lethal rRNA mutations in the peptidyl transferase centre that facilitate the translation of a problematic protein sequence.
Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes
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Combinatorial Synthesis of Genetic Networks
Combinatorial synthesis provides an alternative approach for studying biological networks, as well as an efficient method for producing diverse phenotypes in vivo, with the use of a combinatorial method in Escherichia coli.
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Specialized ribosome system: preferential translation of a single mRNA species by a subpopulation of mutated ribosomes in Escherichia coli.
  • A. Hui, H. D. de Boer
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
A system in which a subpopulation of modified ribosomes are directed to a single mutated mRNA species offers an approach to the study of mutations elsewhere in the 16S-rRNA gene that otherwise would be lethal to the cell.
Mutagenesis at the mRNA decoding site in the 16S ribosomal RNA using the specialized ribosome system in Escherichia coli.
It is concluded that some, but not all, of the nucleotides in the conserved C1400 region play a key role in translation.
Thiamine derivatives bind messenger RNAs directly to regulate bacterial gene expression
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Initiation of Protein Synthesis in Bacteria
A detailed description of current knowledge about the structure, function, and interactions of the individual components involved in bacterial translation initiation is presented and the mechanisms of regulation of the translation initiation event are provided.
Gene regulatory network growth by duplication
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Directed evolution of a genetic circuit
It is demonstrated that a nonfunctional circuit containing improperly matched components can evolve rapidly into a functional one and generated a library of genetic devices with a range of behaviors that can be used to construct more complex circuits.