A nested sequence-specific primer-polymerase chain reaction for the detection of HLA-B*15:02.

Abstract

In this study, we reported a new technique in detecting HLA-B*15:02 by using a nested sequence-specific primer-polymerase chain reaction (SSP-PCR) that can be used on genomic DNA and whole blood for carbamazepine hypersensitivity prediction. We tested a total of 200 blind samples with known human leukocyte antigen (HLA)-B allelic types (44 positive for HLA-B*15:02 and 156 negative for HLA-B*15:02) with this new nested SSP-PCR technique and compared its efficacy to that of commercial sequence-specific oligonucleotide probe-polymerase chain reaction (SSOP-PCR). Using starting materials from DNA and whole blood, we were able to detect HLA-B*15:02 in 44 of our samples correctly. The test is very sensitive and is highly reproducible.

DOI: 10.1111/j.1399-0039.2012.01836.x

Cite this paper

@article{Virakul2012ANS, title={A nested sequence-specific primer-polymerase chain reaction for the detection of HLA-B*15:02.}, author={Supreeya Virakul and Pawinee Kupatawintu and Jeerawat Nakkuntod and Oratai Kangwanshiratada and Tirayut Vilaivan and Nattiya Hirankarn}, journal={Tissue antigens}, year={2012}, volume={79 4}, pages={295-301} }