A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine.

@article{Longstaff2007ANG,
  title={A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine.},
  author={David Gordon Longstaff and Ross C. Larue and Joseph E. Faust and Anirban Mahapatra and Liwen Zhang and Kari B. Green-Church and Joseph Adrian Krzycki},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 3},
  pages={1021-6}
}
Pyrrolysine has entered natural genetic codes by the translation of UAG, a canonical stop codon. UAG translation as pyrrolysine requires the pylT gene product, an amber-decoding tRNA(Pyl) that is aminoacylated with pyrrolysine by the pyrrolysyl-tRNA synthetase produced from the pylS gene. The pylTS genes form a gene cluster with pylBCD, whose functions have not been investigated. The pylTSBCD gene order is maintained not only in methanogenic Archaea but also in a distantly related Gram-positive… CONTINUE READING
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