A native antibody-based mobility-shift technique (NAMOS-assay) to determine the stoichiometry of multiprotein complexes.

@article{Swamy2007ANA,
  title={A native antibody-based mobility-shift technique (NAMOS-assay) to determine the stoichiometry of multiprotein complexes.},
  author={Mahima Swamy and Susana Minguet and Gabrielle Melanie Siegers and Balbino Alarc{\'o}n and Wolfgang Schamel},
  journal={Journal of immunological methods},
  year={2007},
  volume={324 1-2},
  pages={74-83}
}
Characterization of multiprotein complexes (MPCs) is an important step toward an integrative view of protein interaction networks and prerequisite for a molecular understanding of how a certain MPC functions. Here, we present a technique utilizing monoclonal subunit-specific antibodies for an electrophoretic immunoshift assay in Blue Native-gels (NAMOS-assay), which allows the determination of the stoichiometry of MPCs. First, we use the B cell antigen receptor as a model MPC whose… CONTINUE READING

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